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  • Novel assay of antibacterial components in manuka honey using lucigenin-chemiluminescence-HPLC.

Novel assay of antibacterial components in manuka honey using lucigenin-chemiluminescence-HPLC.

Analytica chimica acta (2017-01-14)
Koji Karasawa, Shiomi Haraya, Sachie Okubo, Hidetoshi Arakawa
ABSTRACT

Five components (hydrogen peroxide, methylglyoxal, dihydroxyacetone, fructose and glucose) of New Zealand manuka honey (Leptospermum scoparium) were analyzed using lucigenin chemiluminescence high-performance liquid chromatography (lucigenin-CL-HPLC). We focused on active oxygen species produced from the components in order to easily detect these five components contained in manuka honey. H

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bis(2,4,6-trichlorophenyl) oxalate, BioReagent, suitable for chemiluminescence, ≥99.0% (AT)
Sigma-Aldrich
2-Bromo-4′-nitroacetophenone, 95%