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Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long-Term Functional Stability and Controlled Release of Biomacromolecules.

Advanced healthcare materials (2015-06-20)

Timothy M O'Shea, Matthew J Webber, Alex A Aimetti, Robert Langer

ABSTRACT

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and -controlled release of model enzymes in vitro and in vivo as well as preservation of protein stability under heat and -lyophilization stressors. Strong and -ordered hydrogen bonding interactions within covalently incorporated trehalose hydrogels represent a possible mechanism for protein stabilization.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, SAJ special grade

Sigma-Aldrich

Acetone, for chromatography, ≥99.8%

Sigma-Aldrich

Acetone, for residue analysis, JIS 5000

Sigma-Aldrich

Acetone, suitable for HPLC

Sigma-Aldrich

Hydrochloric acid solution, 6 M

Sigma-Aldrich

Acetone, ≥99.5%, for residue analysis

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, for residue analysis

Sigma-Aldrich

Hydrochloric acid solution, 0.2 M

Sigma-Aldrich

Acetone, for residue analysis, ≥99.5%

Sigma-Aldrich

Hydrochloric acid, SAJ first grade, 35.0-37.0%

Sigma-Aldrich

Hydrochloric acid solution, 0.02 M

Sigma-Aldrich

Hydrochloric acid solution, 0.01 M

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

Sigma-Aldrich

Hydrogen chloride – ethanol solution, 0.1 M in ethanol

Sigma-Aldrich

Hydrochloric acid solution, 12 M

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

Sigma-Aldrich

Hydrochloric acid, JIS special grade, 35.0-37.0%

Sigma-Aldrich

Hydrochloric acid solution, 0.05 M

Sigma-Aldrich

Methanol, suitable for HPLC

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥99% (HPLC), from starch

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

Sigma-Aldrich

Hydrochloric acid solution, 32 wt. % in H₂O, FCC

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, tablet, 1 mg substrate per tablet

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)

Sigma-Aldrich

Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology

Supelco

Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free

Sigma-Aldrich

D-(+)-Trehalose dihydrate, ≥98.5% (HPLC), from Saccharomyces cerevisiae

Sigma-Aldrich

D-(+)-Trehalose dihydrate, from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture