Skip to Content
Merck
  • Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection.

Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection.

International journal of medical microbiology : IJMM (2014-04-24)
Mabel Lum, Renato Morona
ABSTRACT

Shigella infection in epithelial cells induces cell death which is accompanied by mitochondrial dysfunction. In this study the role of the mitochondrial fission protein, Drp1 during Shigella infection in HeLa cells was examined. Significant lactate dehydrogenase (LDH) release was detected in the culture supernatant when HeLa cells were infected with Shigella at a high multiplicity of infection. Drp1 inhibition with Mdivi-1 and siRNA knockdown significantly reduced LDH release. HeLa cell death was also accompanied by mitochondrial fragmentation. Tubular mitochondrial networks were partially restored when Drp1 was depleted with either siRNA or inhibited with Mdivi-1. Surprisingly either Mdivi-1 treatment or Drp1 siRNA-depletion of HeLa cells also reduced Shigella plaque formation. The effect of Mdivi-1 on Shigella infection was assessed using the murine Sereny model, however it had no impact on ocular inflammation. Overall our results suggest that Drp1 and the mitochondria play important roles during Shigella infection.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
p-Phenylenediamine, ≥99.0% (GC/NT)
Sigma-Aldrich
p-Phenylenediamine, 98% (GC)
Sigma-Aldrich
Mdivi-1, ≥98% (HPLC), powder
Sigma-Aldrich
Necrostatin-1, ≥98% (HPLC)
Lysine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Supelco
4-tert-Octylphenol monoethoxylate solution, 10 μg/mL in acetone, analytical standard
Sigma-Aldrich
p-Phenylenediamine, sublimed, ≥99%
Sigma-Aldrich
MISSION® esiRNA, targeting human DNM1L
Sigma-Aldrich
Anti-Caspase 3 Antibody, active (cleaved) form, Chemicon®, from rabbit
Sigma-Aldrich
Anti-DNM1L antibody produced in rabbit, affinity isolated antibody
Supelco
L-Lysine monohydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
L-Lysine monohydrochloride, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
L-Lysine monohydrochloride, SAJ special grade, ≥99.0%
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Dnm1l
Supelco
L-Lysine hydrochloride solution, 100 mM amino acid in 0.1 M HCl, analytical standard
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
L-Lysine monohydrochloride, reagent grade, ≥98% (HPLC)
Sigma-Aldrich
IM-54, ≥98% (HPLC)
Sigma-Aldrich
L-Lysine monohydrochloride, from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
Sigma-Aldrich
L-Lysine monohydrochloride, BioUltra, ≥99.5% (AT)