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N9876

Sigma-Aldrich

4-Nitrophenyl butyrate

lipase substrate, chromogenic, esterase substrate, ≥98%, liquid

Synonym(s):

p-Nitrophenyl butyrate, Butyric acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C10H11NO4
CAS Number:
2635-84-9
Molecular Weight:
209.20
Beilstein:
1879729
MDL number:
MFCD00047734
UNSPSC Code:
12352204
PubChem Substance ID:
24897915
NACRES:
NA.83

product name

4-Nitrophenyl butyrate, ≥98%

Assay

≥98%

form

liquid

density

1.19 g/mL at 20 °C (lit.)

storage temp.

−20°C

SMILES string

CCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C10H11NO4/c1-2-3-10(12)15-9-6-4-8(5-7-9)11(13)14/h4-7H,2-3H2,1H3

InChI key

DVDUMIQZEUTAGK-UHFFFAOYSA-N

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General description

4-Nitrophenyl butyrate is a substrate for esterase, and lipase enzyme. Hydrolysis of 4-nitrophenyl butyrate by these enzymes releases the chromophore, 4-nitrophenolate which is spectrophotometrically analysed at 415 nm.

Application

4-nitrophenyl butyrate has been used:
  • as a substrate for esterase activity in monocyte-derived macrophage cell line in response to polyethylene
  • as a substrate for mycobacterial phospholipase A and mycobacterial cutinase activity
  • as a substrate to determine the effect of buffers and solvents on human carboxylesterase

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Monitoring lipase/esterase activity by stopped flow in a sequential injection analysis system using p-nitrophenyl butyrate
Pliego J, et al.
Sensors, 15(2), 2798-2811 (2015)
Li-Chun Wang et al.
Journal of food and drug analysis, 26(3), 973-984 (2018-07-07)
Obesity is associated with higher risks of developing diabetes and cardiovascular disease. Green tea, rich in polyphenolic compounds such as epigallocatechin gallate (EGCG) and epigallocatechin (EGC), has been shown to display anti-obesity effects. Houttuynia cordata leaves have also been shown
Nathalia S Rios et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(9), 741-747 (2019-06-16)
The lipase from Pseudomonas fluorescens (PFL) has been immobilized on glyoxyl-octyl agarose and compared to the enzyme immobilized on octyl-agarose. Thus, PFL was immobilized at pH 7 on glyoxyl-octyl support via lipase interfacial activation and later incubated at pH 10.5 for 20 h
Differential response to chemically altered polyethylene by activated mature human monocyte-derived macrophages
Xing S, et al.
Biomaterials, 23(17), 3595-3602 (2002)
Jose J Virgen-Ortíz et al.
Enzyme and microbial technology, 96, 30-35 (2016-11-23)
Lipase B from Candida antarctica (CALB) has been physically immobilized on octyl-agarose via interfacial activation. The incubation of the enzyme in 80% ethanol at pH 5 and 25°C has not significant effect on enzyme activity. Moreover, the hydrolysis of 100mM
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