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L3916

Sigma-Aldrich

L-Lactic Dehydrogenase from bovine heart

1000 units/mL

Synonym(s):

Lactate, (S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD

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About This Item

CAS Number:
9001-60-9
Enzyme Commission number:
1.1.1.27 (BRENDA, IUBMB)
EC Number:
232-617-8
MDL number:
MFCD00131466
UNSPSC Code:
12352204
NACRES:
NA.54

form

solution

specific activity

1000 units/mL

storage temp.

2-8°C

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General description

LDH (lactic dehydrogenase) is a glycolytic enzyme. It is found in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It comprises of five isoenzyme forms. LDH possess a tetrameric structure.

Application

L-Lactic Dehydrogenase from bovine heart has been used:
  • as a reference standard to measure the LDH (lactic dehydrogenase)
  • to quench protein-bound NADH in cells
  • to measure the lactate released by the cells in suspension

For use in enzymatic determination of lactate or pyruvate.

Biochem/physiol Actions

LDH (lactic dehydrogenase) helps in the reversible conversion of lactate to pyruvate.
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids.

Unit Definition

One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C.

Physical form

Suspension in 2.2 M ammonium sulfate

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mitochondrial angiotensin receptors in dopaminergic neurons. Role in cell protection and aging-related vulnerability to neurodegeneration
Valenzuela R, et al.
Cell Death & Disease, 7(10), e2427-e2427 (2016)
NADH fluorescence lifetime is an endogenous reporter of alpha-synuclein aggregation in live cells
Plotegher N, et al.
Faseb Journal, 29(6), 2484-2494 (2015)
Hsiao Ju Chiang et al.
Nature methods, 20(2), 248-258 (2023-01-20)
The expansion of fluorescence bioimaging toward more complex systems and geometries requires analytical tools capable of spanning widely varying timescales and length scales, cleanly separating multiple fluorescent labels and distinguishing these labels from background autofluorescence. Here we meet these challenging
Culture Expansion in Low-Glucose Conditions Preserves Chondrocyte Differentiation and Enhances Their Subsequent Capacity to Form Cartilage Tissue in Three-Dimensional Culture
HeywoodHannah K, et al.
BioResearch Open Access, 2484-2494 (2014)
Circulating biomarkers in malignant melanoma
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of L-Lactic Dehydrogenase (EC 1.1.1.27)

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

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