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BSAV-RO

Roche

Bovine Serum Albumin

≥98.5%, New Zealand origin

Synonym(s):

Bovine Serum Albumin Fraction V

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About This Item

UNSPSC Code:
12352202

biological source

bovine

Quality Level

Assay

≥98.5%

form

lyophilized

mol wt

Mr =68 kDa

packaging

pkg of 1 kg (10735108001)
pkg of 100 g (10735086001)
pkg of 50 g (10735078001)
pkg of 500 g (10735094001)

manufacturer/tradename

Roche

origin

New Zealand origin

technique(s)

ELISA: suitable
Southern blotting: suitable
western blot: suitable

impurities

<0.0003 Lithium (Flame photometry)
<0.001 Iron (bathophenanthraline)
<0.002 P (inorganic)
<0.003 Heavy metals (as Pb)
<0.003 Magnesium (xylidyl blue)
<0.005 Glycerol (enzyme)
<0.006 potassium (Flame photometry)
<0.02 Calcium ( o-cresolphthalein)
<0.05 glucose (enzyme)
<0.1 L-lactate
<0.15 chloride (mercurom)
<0.5 Sodium (flame photometry)
<100 microorganisms (organism/g)
<5 water

absorption

≤0.4 at 550 nm

UniProt accession no.

foreign activity

Microorganisms <100 (Organism/g)

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

For standard applications.

Application

Bovine Serum Albumin Fraction V is suitable for standard applications such as:
  • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
  • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
  • Reducing agent
  • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
  • Media supplement for media that require addition of protein
  • Carrier protein
  • Standard protein in gel electrophoresis and protein determination

Quality

Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

Preparation Note

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Laurent Berchebru et al.
Journal of microbiological methods, 105, 141-145 (2014-07-20)
Test and evaluation of engineered biothreat agent detection systems ("biodetectors") are a challenging task for government agencies and industries involved in biosecurity and biodefense programs. In addition to user friendly features, biodetectors need to perform both highly sensitive and specific
Mariko Kuse et al.
The Journal of reproduction and development, 59(4), 346-352 (2013-04-09)
Cortisol (Cr), the most important glucocorticoid (GC), is well known to suppress uterine prostaglandin F2α (PGF) production. However, the details of the regulatory mechanisms controlling the cyclic changes in endometrial PGF production remain unclear. Here we investigated the expression of
Paulina Krzysica et al.
Animals : an open access journal from MDPI, 12(21) (2022-11-12)
Cytokines like interferon (IFN)-γ, interleukin (IL)-2, IL-6, IL-10, and IL-12p40 are important biomarkers for characterizing the nature and strength of immune responses. It is important to be able to quantify the cytokines at the protein level in biological samples. Quantification
Leonie Kollenstart et al.
Scientific reports, 11(1), 12795-12795 (2021-06-19)
The collection of known posttranslational modifications (PTMs) has expanded rapidly with the identification of various non-acetyl histone lysine acylations, such as crotonylation, succinylation and butyrylation, yet their regulation is still not fully understood. Through an unbiased chromatin immunoprecipitation (ChIP)-based approach
Francis K Y Siu et al.
Nature protocols, 3(1), 51-58 (2008-01-15)
Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor.

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