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  • Linear retention index approach applied to liquid chromatography coupled to triple quadrupole mass spectrometry to determine oxygen heterocyclic compounds at trace level in finished cosmetics.

Linear retention index approach applied to liquid chromatography coupled to triple quadrupole mass spectrometry to determine oxygen heterocyclic compounds at trace level in finished cosmetics.

Journal of chromatography. A (2021-05-27)
Adriana Arigò, Paola Dugo, Francesca Rigano, Luigi Mondello
ABSTRACT

In the European Union, cosmetic products are regulated by the Cosmetics Regulation (EC) No. 1223/2009. The Analytical Working Group of the International Fragrance Association (IFRA) suggested that a new sensitive analytical method is needed to determine psoralens in finished cosmetic products. This research provides an HPLC-MS/MS method for the quality control of 20 furocoumarins, 8 coumarins and 7 polymethoxyflavones in cosmetics. Thanks to the high sensitivity of the tandem mass spectrometry detection in Multiple Reaction Monitoring mode, psoralens contained in trace have been quantified in different products. The Limits of Quantifications were in the range 0.3-74 μg L-1. A reliable identification was achieved combining the Linear Retention Index (LRI) system with the MS and MS/MS libraries. In particular, the attribution was based on the spectra similarity filtered by the LRI parameter. The robustness and reproducibility of the LRI approach was demonstrated by the comparison of the HPLC-MS/MS results here reported with those obtained in our previous study, by using an HPLC-PDA system. This method could be taken into account for quality control of furocoumarins in cosmetics, and by the main associations, such as IFRA, for the purpose of issuing new opinions.

MATERIALS
Product Number
Brand
Product Description

Supelco
Bergamottin, analytical standard
Supelco
Acetophenone, analytical standard
Trioxsalen, phyproof® Reference Substance
Phellopterin, phyproof® Reference Substance
Biacangelicol, phyproof® Reference Substance
Supelco
5-Geranyloxy-7-methoxycoumarin, analytical standard
Supelco
Propiophenone, analytical standard
Isobergapten, phyproof® Reference Substance
5-Demethylnobiletin, phyproof® Reference Substance
Oxypeucedanin hydrate, phyproof® Reference Substance
Supelco
Butyrophenone, analytical standard
Supelco
Bergapten, analytical standard
Supelco
8-Methoxypsoralen, analytical standard
Supelco
Ascentis® Express C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Cnidilin, analytical standard
Supelco
Tetra-O-methylscutellarein, analytical standard
Supelco
Cnidicin, analytical standard
Supelco
Meranzin hydrate, analytical standard
Supelco
Meranzin, analytical standard
Oxypeucedanin, phyproof® Reference Substance
Gardenin B, phyproof® Reference Substance
(±)-Heraclenin, phyproof® Reference Substance
Byakangelicin, phyproof® Reference Substance