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Discovery® HS C18 (3 µm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® HS C18 HPLC Column, 3 μm particle size, L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

20% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 2.1 mm

surface area

300 m2/g

surface coverage

3.2 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

3 μm

pore size

120 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

Discovery® HS C18 HPLC column (L × I.D. 15 cm × 2.1 mm) is found to be stable under acidic and basic conditions.

Features and Benefits

  • Stable, low bleed for LC-MS applications
  • Scalable from analytical to preparatory
  • Highly stable to ensure excellent run-to-run and lot-to-lot reproducibility
  • Higher hydrophobicity for better resolution of difficult analytes
   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Abad Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 878(28), 2782-2788 (2010-09-11)
A rapid, inexpensive, sensitive and specific HPLC-ECD method for the determination of lipoic acid in human plasma was developed and validated over the linearity range of 0.001-10μg/ml using naproxen sodium as an internal standard (IS). Extraction of lipoic acid and
Fazli Khuda et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 944, 114-122 (2013-12-10)
A simple, specific, precise and rapid RP-HPLC-UV method was developed for simultaneous determination of lumefantrine and its metabolite desbutyl lumefantrine in human plasma. Experimental parameters were optimized and the method was validated according to standard guidelines. The method showed adequate
Lucie Zelena et al.
Journal of pharmaceutical and biomedical analysis, 137, 70-77 (2017-01-17)
Efavirenz is an antiretroviral drug used in the treatment of HIV-positive patients. A simple, fast and sensitive high-performance liquid chromatography (HPLC) method was developed in order to determine efavirenz in three types of samples provided from pharmacokinetic studies. The analysis
Awantika Singh et al.
Rapid communications in mass spectrometry : RCM, 29(6), 485-496 (2015-07-15)
Adhatoda beddomei and Adhatoda vasica are popular Ayurvedic medicinal plants in India, belonging to the family Acanthaceae. Tricyclic quinazoline alkaloids are found to be the most abundant in these plants which are responsible for broad-spectrum medicinal properties. This study aims
Jolanta Flieger et al.
Journal of separation science, 34(7), 733-739 (2011-03-02)
Highly hydrophilic compounds belonging to biogenic amines were analysed in the reversed-phase system, modified with the addition of ionic liquids: 1-ethyl-3-methyl-imidazolium hexafluorophosphate (EMIM PF(6)) and chaotropic salt NaPF(6) on Discovery HS C18 column at acidic conditions. The effect of the

Protocols

HPLC Analysis of Polyphenols in Nero d'Avola Red Wine on Discovery® HS C18 (UV 280 nm)

Related Content

HPLC DiscoveryBIO HS C18 products. Available in 3µm, 5µm and 10µm particle sizes.

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