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Key Documents

07-1398

Sigma-Aldrich

Anti-phospho-Akt (Thr308) Antibody

from rabbit

Synonym(s):

Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, canine

species reactivity (predicted by homology)

Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology), human (based on 100% sequence homology), dog (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG

suitability

not suitable for Western blot

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pThr308)

Gene Information

bovine ... Akt1(280991)
human ... AKT1(207)
rat ... Akt1S1(292887)

General description

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

Immunogen

Epitope: Thr308 (Catalytic Domain)
KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1. Human and mouse Akt2 shares 11/12 amino acids and rat Akt3 shares 10/12 amino acids with the peptide immunogen sequence.

Application

Immunohistochemistry only

Western Blot Analysis:
Not recommended for WB as it gives multiple bands. Please refer to 05-802R
This Anti-phospho-Akt (Thr308) Antibody is validated for use in IH(P) for the detection of phospho-Akt (Thr308).

Quality

Immunohistochemistry Analysis:
Antibody diluted to 1:500, IHC-Select Detection with HRP-DAB.

Target description

60 kDa

Physical form

Antigen affinity purified against both the phosphorylated and the non-phosphorylated peptides of Akt (Thr308).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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