05-201
Anti-Fas Antibody (human, activating), clone CH11
clone CH11, Upstate®, from mouse
Synonym(s):
APO-1 cell surface antigen, CD95 antigen, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily, member 6
About This Item
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biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
CH11, monoclonal
purified by
affinity chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... FAS(355)
General description
Biological Activity
The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.
Specificity
Immunogen
Application
Apoptosis & Cancer
Apoptosis - Additional
0.5-2 μg/mL of a previous lot detected Fas in a Raji cell lysate.
Immunocytochemistry:
5-10 μg/mL of a previous lot detected Fas on HeLa cells fixed with 4% formalin/2% acetic acid.
Flow cytometry:
A previous lot of was tested by an independent laboratory using 20 μg/mL of anti-Fas, clone CH11 (Yonehara, S., 1989; Kobayashi, N., 1990).
Quality
Apoptosis Assay Analysis:
15-20 µg/mL of this lot maximally induced apoptosis of human Jurkat cells with 83% mortality after 24 hours of treatment.
Target description
Physical form
Storage and Stability
Analysis Note
Human liver tumor, human breast tumor or Jurkat whole cell lysate, Raji cell lysate.
Other Notes
Legal Information
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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