Technical Bulletins
Bacterial Glycerol Stock (SHCLNG)
Bacterial cultures may be amplified from the glycerol stocks for use in purification of shRNA plasmid DNA. Subsequently, target cell lines may be transfected with the purified plasmid for transient or stable gene silencing (puromycin selection). In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
Individual shRNA clones are provided as a frozen bacterial glycerol stock (E. coli) containing Terrific Broth (TB), carbenicillin at 100 µg/mL and 10% glycerol.
Download the SHCLNG Technical Bulletin (583 kb PDF)
Purified Plasmid DNA Format (SHCLND)
We offer the highest quality purified plasmid DNA for downstream transfection and lentiviral packaging. DNA purified with our 96-well DNA purification system, developed specifically for the TRC library, yields the highest quality lentiviral particles.
Purified plasmid may be directly used in transient or stable gene silencing (puromycin selection). In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
Individual constructs are provided as frozen stocks containing 1 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 20 ng/µL. See gene family set technical bulletins for specifications, inquire for library specifications.
Download the SHCLND Technical Bulletin (561 kb PDF)
Lentiviral Format (SHCLNV)
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the lentiviral transduction particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. The lentiviral transduction particles are titered via a p24 antigen ELISA assay and pg/mL of p24 are then converted to transducing units per mL using a conversion factor.
Individual constructs are provided as a 200 µL frozen stock containing a minimum of 1.0 x 106 TU/mL (via p24 assay) in Dulbecco’s Modified Eagle’s Medium with 10% heat-inactivated fetal bovine serum and penicillin-streptomycin. Our average titers for individual clone orders are routinely greater than 1.0 x 107 TU/mL (via p24 assay). See gene family set technical bulletins for specifications, inquire for library specifications.
MISSION® Control Vectors
Purified plasmid control vectors may be directly used in transient or stable gene silencing (puromycin selection). In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. Visit our controls webpage for more information on specific controls.
Individual control vectors are provided as 10 µg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/µL.
Download the MISSION® Control Vectors Technical Bulletin (226 Kb PDF)
MISSION® Control Transduction Particles
We offer a variety of positive, negative, and transduction controls in lentiviral format. Additionally, our most popular controls are offered in high titer format. Visit our controls webpage for more information on specific controls.
Standard titer control transduction particles are provided as 200 µL of 106 TU/mL (via p24 titering assay). High titer control transduction particles are provided as 10 x 20 µL aliquots of 109 TU/mL (via p24 titering assay).
MISSION® Lentiviral Packaging Mix (SHP001)
The MISSION® Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene. It is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications.
The MISSION® Lentiviral Packaging Mix is available in two package sizes: SHP001-0.25 mL and SHP001-1.7 mL.
MISSION® 3'UTR Lenti GoClone™
The 3' untranslated regions (3'UTRs) of mRNA transcripts control many aspects of post-transcriptional regulation of genes. 3'UTRs are involved in regulating transcript stability, translational efficiency, and localization in the cell. 3'UTRs are also the predominant target sequences for endogenous microRNAs (miRNA) or small interfering RNAs (siRNA).
We have teamed up with SwitchGear Genomics to offer you the MISSION® 3'UTR Lenti
GoClone™ collection, which is a genome-wide collection of cloned human 3'UTRs. Each viral vector expresses an optimized luciferase reporter gene (RenSP) fused to a 3’UTR sequence.
Download the MISSION® 3'UTR Lenti GoClone™ Technical Bulletin (154 Kb PDF)
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