HPLC Analysis of Epinephrine, Epinastine, and Benzalkonium Chlorides on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 50 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

ESI(+), XIC m/z 304 & 332 (BAC), 250 (epinastine), 180 (epinephrine)

injection

10 μL

sample

10 mg/L ea. in acetonitrile