- A new dark quencher for use in genetic analysis.
A new dark quencher for use in genetic analysis.
Chemical communications (Cambridge, England) (2003-05-15)
Jonathan P May, Lynda J Brown, Ivo Rudloff, Tom Brown
PMID12744324
摘要
A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5.