Antibacterial multilayer films fabricated by LBL immobilizing lysozyme and HTCC on nanofibrous mats.

Cellulose acetate nanofibrous mats were prepared by electrospunning technique. Positively charged and negatively charged composites were alternately deposited on negatively charged CA mats via layer-by-layer self-assembly technique. The morphology and inhibition rate of samples were investigated by regulating number of deposition bilayers and composition of outermost layer. Field emission scanning electron microscopy images indicated that the average diameter of fibers was increased with increasing the number of coating bilayers. Additionally, the catalytic activity of immobilized LY was measured and results showed "layer-by-layer alternative oscillation" phenomenon. The results of antibacterial assay indicated that (LY-HTCC/ALG)(10.5) films coating had the best inhibitory effect.