跳转至内容
Merck
  • Analysis of the polyphenols content in medicinal plants based on the reduction of Cu(II)/bicinchoninic complexes.

Analysis of the polyphenols content in medicinal plants based on the reduction of Cu(II)/bicinchoninic complexes.

Journal of agricultural and food chemistry (2009-11-11)
Daniele Cestari Marino, Larissa Zuppardo Lacerda Sabino, José Armando, Andrea De Andrade Ruggiero, Horacio Dorigan Moya
摘要

A spectrophotometric method is proposed for the determination of the polyphenols content in aqueous extracts of plants. The method is based on the reduction of Cu(II) to Cu(I) by polyphenols, in the presence of bicinchoninic acid in a buffered medium (ammonium acetate, pH 7.0) with the formation of Cu(I)/BCA complexes. A calibration curve of absorbance (at 558 nm) vs tannic acid concentration is linear (r = 0.995; n = 7) with tannic acid from 0.1 to 0.7 micromol L(-1). The limit of detection and relative standard deviation were 40 nmol L(-1) (99% confidence level) and 3.8% (0.4 micromol L(-1) tannic acid, n = 7), respectively. For the aqueous extracts of Hamamelis virginiana L., Maytenus ilicifolia Mart. ex Reissek, Hydrocotyle bonariensis Lam, Annona muricata L., Myrciaria cauliflora (Mart.) O. Berg., Caesearia sylvestris Sw., Schinus terebinthifolia (Raddi), and Stryphnodendron adstringens (Mart.) Coville, the total polyphenol contents, expressed as tannic acid, were 3.5, 1.3, 2.0, 3.1, 15.4, 3.1, 9.1, and 6.9%, respectively.

材料
货号
品牌
产品描述

Sigma-Aldrich
2,2′-联喹啉-4,4′-二羧酸, ≥90% (TLC)