跳转至内容
Merck
  • Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells.

Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells.

Journal of dentistry (2020-10-18)
Liming Jiang, Wayne Nishio Ayre, Genevieve E Melling, Bing Song, Xiaoqing Wei, Alastair James Sloan, Xu Chen
摘要

This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration. 0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. "Osteodentine" differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining. The ELISA results showed that liposomal TGF-β1 achieved a controlled and prolonged release over time. The MTT results demonstrated that the liposomes (100 μg/mL) were not cytotoxic to the cells. Liposomal TGF-β1 up-regulated the expression of "osteodentine" markers, RUNX-2, DMP-1 and DSPP, in hDPSCs after 7 days of treatment and resulted in the accumulation of mineralised nodules. This study indicated that liposomes are an effective carrier for delivering TGF-β1 over time. Liposomal TGF-β1 promoted dentinogenesis and increased mineralisation in hDPSCs. This highlights the potential of liposomal TGF-β1 for future use in dentine-pulp regeneration. Liposomal TGF-β1 may be used as a synergist for promoting dentine-pulp regeneration of immature permanent teeth or as a pulp capping agent for inducing reparative dentine formation.

材料
货号
品牌
产品描述

Sigma-Aldrich
人DMP1 /牙本质基质酸性磷酸蛋白1 ELISA试剂盒, for serum, plasma, cell culture supernatants and urine