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  • Ribosomal Protein Conferring Sensitivity to the Antibiotic Spectinomycin in Escherichia coli.

Ribosomal Protein Conferring Sensitivity to the Antibiotic Spectinomycin in Escherichia coli.

Science (New York, N.Y.) (1969-07-04)
A Bollen, J Davies, M Ozaki, S Mizushima
摘要

Reconstitution of 30S ribosomal particles from 16S ribosomal RNA and total proteins, or from core proteins and split proteins obtained from the ribosomes of strains of Escherichia coli sensitive to and resistant to spectinomycin, shows that the split protein fraction determines the response of polypeptide synthesis in virto to spectinomycin. Reconstitution of active particles in the presence of isolated split proteins allowed the identification of the single split protein responsible for spectinomycin sensitivity.

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Spectinomycin ready made solution, 100 mg/mL in DMSO/H2O, 1:1, Cell culture tested