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生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
4B20, monoclonal
recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 76.62 kDa
observed mol wt ~100 kDa
物種活性
bovine, human
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
bovine ... Gab1(540085)
human ... GAB1(2549)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 4B20 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects GRB2-associated-binding protein 1 (Gab1). It targets an epitope within 30 amino acids from the C-terminal region.
免疫原
KLH-conjugated Linear Peptide corresponding to 30 amino acids from the C-terminal region of Human Gab1.
應用
Anti-Gab1, clone 4B20 ZooMAb, Cat. No. ZRB1348, is a recombinant Rabbit monoclonal antibody that specifically targets GRB2-associated-binding protein 1 (Gab1) and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Gab1 in fetal bovine heart endothelial (FBHE) cell lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Gab1 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Gab1 peptide with a KD of 4.0 x 10-6 in affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Gab1 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Gab1 peptide with a KD of 4.0 x 10-6 in affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
GRB2-associated-binding protein 1 (UniProt: Q13480; also known as GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1; Gab1) is encoded by the GAB1 gene (Gene ID: 2549) in human. Gab1 is a scaffolding protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. It recruits PI-3 kinase and other effector proteins in response to the activation of many receptor tyrosine kinases (RTKs). Gab1 can be phosphorylated on at least 16 sites by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta, hepatocyte growth factor/scatter factor receptor, and epidermal growth factor receptor. Phosphorylation of Gab1 on tyrosine 627 and tyrosine 659 is shown to be critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. Tyrosine phosphorylation of Gab1 mediates interaction with several proteins that contain SH2 domains. Gab1 plays important roles in embryonic development and cell migration and is shown to be a critical component in the interaction between focal adhesion kinase and Met in cancer cell invasion. Gab1 is also reported to be a major target in linoleic acid (LA)-induced enhancement of tumorigenesis. Mutations in GAB1 gene are known to cause autosomal recessive deafness that is characterized by severe to profound hearing loss resulting from damage to the neural receptors of the inner ear or the nerve pathways to the brain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mattoon, DR., et al. (2004). BMC Biol. 2; 24; Mouradian, M., et al. (2014). Prostaglandins Leukot. Essent. Fatty Acids. 90(4); 105-115).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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