SRP0733

PCTK2 (CDK17)/CYCLINY, Active

PRECISIO^® Kinase, recombinant

• 分類程式碼代碼: 12352202
• NACRES: NA.77

属性

• 重組細胞: expressed in baculovirus infected Sf9 cells
• 化驗: 70-95% (SDS-PAGE)
• 形狀: frozen liquid
• 比活性: 15-25 nmol/min·mg (radiometric)
• 分子量: ~64 kDa (Cyclin Y); ~88 kDa (PCTK2)
• 濃度: 0.1 μg/μL
• 登錄號: NM_002595; NM_145012
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... CCNY(219771) , CDK17(5128)

说明

應用

Useful for kinase assay and Western blot.

生化／生理作用

Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons ⁽¹⁾. The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins ⁽²⁾.

重構

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

儲存和穩定性

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

法律資訊

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable