推荐产品
等級
for molecular biology
形狀
buffered aqueous glycerol solution
濃度
10,000 units/mL
運輸包裝
wet ice
儲存溫度
−20°C
正在寻找类似产品? 访问 产品对比指南
特異性
识别序列： 5′-G/AATTC-3′
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
切割结果：1 μgλ DNA 底物的 2-10 倍 EcoR I 过度消化导致 100% 切割
热灭活： 65°C，20 min。
應用
Eco RI 是一种限制性内切酶，在分子生物学应用中用于切割识别站点 5′ 的 DNA-G/AATTC-3′，产生带有 5′ 的片段-粘性终点。
其他說明
提供 10x 限制性内切酶缓冲液 SH (B3657)。
备注： 避免次优反应条件，如低盐、高 pH 值 ( 8.0) 和高甘油 ( 5%)，因为它们将改变 EcoRI 特异性并沉淀星号活性。
單位定義
一个单位表示在37°C下1小时内，在总体积为50 ml的1x限制酶消化缓冲液SH中完全切割1 mg λDNA的酶活性。1 mg pBR322 DNA可通过2个单位的EcoR I完全消化。
外觀
10 mM Tris-HCl 溶液，pH 值 7.2、1 mM EDTA、200 mM NaCl、0.5 mM 二硫赤藓糖醇、50% 甘油 (v/v)、0.2%Triton X-100 (v/v)，4°C
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
J Hedgpeth et al.
Proceedings of the National Academy of Sciences of the United States of America, 69(11), 3448-3452 (1972-11-01)
The sequence of DNA base pairs adjacent to the phosphodiester bonds cleaved by the RI restriction endonuclease in unmodified DNA from coliphage lambda has been determined. The 5'-terminal nucleotide labeled with (32)P and oligonucleotides up to the heptamer were analyzed
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
Amal M El-Shehaby et al.
Scandinavian journal of clinical and laboratory investigation, 73(1), 75-81 (2012-12-04)
Left ventricular hypertrophy (LVH) is a common manifestation of cardiovascular disease and has an important prognostic value in patients with end-stage renal disease (ESRD). Vitamin D receptor (VDR) has been intensively investigated, and one of these (BsmI) already has been
C Kessler et al.
Gene, 92(1-2), 1-248 (1990-08-16)
The properties and sources of all known class-I, class-II and class-III restriction endonucleases (ENases) and DNA modification methyltransferases (MTases) are listed and newly subclassified according to their sequence specificity. In addition, the enzymes are distinguished in a novel manner according
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门