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應用
可用于通过 O′Brien, W., Anal. Biochem., FB76, 423 (1976) 介绍的方法进行焦磷酸盐测定。
生化／生理作用
Fructose-1,6-bisphosphatase (FBP) is an important enzyme in glucose metabolism. It catalyzes the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate and inorganic phosphate. Fructose-6-phosphate kinase converts fructose-6-phosphate into fructose 1,6-bisphophate in the rate limiting step of the glycolysis cycle.
其他說明
这两种焦磷酸依赖性果糖-6-磷酸激酶的区别在于它们对生理活化剂果糖 2,6-二磷酸 [Fru (2,6)-P2]的反应不同。据报道，来自费氏丙酸杆菌的酶 (F8381) 不受 Fru (2,6)-P2 影响。而来自马铃薯块茎的酶 (F2258) 则可被 Fru (2,6)-P2 激活（在 5.5nM Fru (2,6)-P2 条件下，半最大活化马铃薯块茎酶）。
單位定義
在 pH 7.4 和 30°C 下，一单位每分钟可将 1.0μmol 的焦磷酸盐和果糖-6-磷酸转化为果糖-1,6-二磷酸和无机磷酸盐。
外觀
含咪唑盐和稳定剂
訊號詞
Danger
危險聲明
危險分類
Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1C
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
其他客户在看
Corrigendum: Online Pyrophosphate Assay for Analyzing Adenylation Domains of Nonribosomal Peptide Synthetases.
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200381-e202200381 (2022-07-28)
Daniel L Machell et al.
Chembiochem : a European journal of chemical biology, 23(18), e202200325-e202200325 (2022-07-26)
Detection of pyrophosphate is important in quantifying enzyme activity, particularly adenylation domain activity during non-ribosomal peptide synthesis. The previous development of an enzyme coupled PPi /NADH assay allowed the measurement of such activity in an online fashion using commercially available
B L Bertagnolli et al.
Biochemistry, 23(18), 4101-4108 (1984-08-28)
Inorganic pyrophosphate dependent D-fructose-6-phosphate 1-phosphotransferase from Propionibacterium freudenreichii was purified to apparent homogeneity by the criterion of silver staining on sodium dodecyl sulfate (SDS) gels. In the direction of phosphorylation of fructose 6-phosphate (F6P), an intersecting initial velocity pattern is
T H Nielsen et al.
Planta, 214(1), 106-116 (2002-01-05)
The role of pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) in developing leaves was studied using wild-type tobacco (Nicotiana tabacum L.) and transformants with decreased expression of PFP. (i) The leaf base, which is the youngest and most actively growing area of the leaf
Florencio E Podestá et al.
Archives of biochemistry and biophysics, 414(1), 101-107 (2003-05-15)
The intrinsic fluorescence of potato tuber pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) was used as an indicator of conformational changes due to ligand binding. Binding of the substrates and the allosteric activator fructose-2,6-bisphosphate was quantitatively compared to their respective kinetic effects on enzymatic
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