生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen 60 kDa (additional band at 50 kDa representing degradation products)
物種活性
Arabidopsis sp.
濃度
~2 mg/mL
技術
western blot: 1-2 μg/mL using leaves extract (cytosolic fraction) of transgenic Arabidopsis seedlings expressing YFP-tagged AtEB1b
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
Arabidopsis thaliana ... ATEB1A(823923) , ATEB1B(836370)
特異性
Recognizes AtEB1b fusion protein expressed in transgenic Arabidopsis plants.
免疫原
synthetic peptide corresponding to amino acids 205-221 located in a region near the C-terminus of Arabidopsis AtEB1b, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in AtEB1a and AtEB1c.
應用
Anti-AtEB1b (C-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using leaves extract (cytosolic fraction) of transgenic Arabidopsis seedlings expressing YFP-tagged AtEB1b.
生化／生理作用
End-binding 1 (EB1) proteins are plus-end-tracking proteins that are specifically localized to growing microtubule plus ends that regulate microtubule and interact with intracellular targets. The plant-specific EB1 subtype functions on the spindle microtubules by accumulating in the prophase nucleus. The microtubule-end-binding protein expressed in Arabidopsis thaliana cells (is called as AtEB1a) is fused with green fluorescent protein (GFP) resulting in labelling of spindle poles. During interphase, AtEB1a-GFP labels the microtubule plus end and also marks the minus end. The microtubule associated protein END BINDING 1b (EB1b) is involved as mutants and dampens root responses to touch and enhances gravitropism. In animals, EB1 proteins have acidic C-terminal tails that might induce an auto-inhibitory conformation.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Shinichiro Komaki et al.
Journal of cell science, 123(Pt 3), 451-459 (2010-01-14)
End-binding 1 (EB1) proteins are evolutionarily conserved plus-end-tracking proteins that localize to growing microtubule plus ends where they regulate microtubule dynamics and interactions with intracellular targets. Animal EB1 proteins have acidic C-terminal tails that might induce an autoinhibitory conformation. Although
Jordi Chan et al.
Nature cell biology, 5(11), 967-971 (2003-10-15)
In plants, it is unclear how dispersed cortical microtubules are nucleated, polarized and organized in the absence of centrosomes. In Arabidopsis thaliana cells, expression of a fusion between the microtubule-end-binding protein AtEB1a and green fluorescent protein (GFP) results in labelling
Laura Gleeson et al.
Plant science : an international journal of experimental plant biology, 187, 1-9 (2012-03-13)
The ability of roots to navigate around rocks and other debris as they grow through the soil requires a mechanism for detecting and responding to input from both touch and gravity sensing systems. The microtubule associated protein END BINDING 1b
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