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Merck

C2230

Sigma-Aldrich

天然腔肠素

solid

别名:

3,2-二氢-2-(对羟基苄基)-6-(对羟基苯基)-8-苄基咪唑并 \ [1,2-a] 吡嗪-3-酮, CLZN

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About This Item

经验公式(希尔记法):
C26H21N3O3
CAS号:
分子量:
423.46
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:
NACRES:
NA.32

化驗

≥95% (TLC)

形狀

solid

溶解度

methanol and ethanol: soluble

儲存溫度

−20°C

SMILES 字串

Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1

InChI

1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2

InChI 密鑰

YHIPILPTUVMWQT-UHFFFAOYSA-N

一般說明

腔肠素是萤光素分子,可被多种萤光素酶利用,如 海肾属刺虾属绿叶水母中存在的荧光素酶。

應用

天然腔肠素用于:
  • 水母发光蛋白测定
  • 在 COS-7 细胞中使用水母发光蛋白测量离子霉素或 PMA 诱导的细胞内钙离子浓度
  • 在生物发光成像中分析病毒依赖性荧光素酶活性的存在

生化/生理作用

当 Ca 2 + 与复合物结合时,水母发光蛋白复合物的发光基团被氧气氧化并在 465 nm 处发光;用于测量细胞内 Ca 2 + 浓度,灵敏度高,动态范围大。

注意

避免光照

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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