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Merck

B5283

Sigma-Aldrich

Anti-Rabbit IgG (γ-chain specific)−Biotin antibody, Mouse monoclonal

clone RG-96, purified from hybridoma cell culture

别名:

Monoclonal Anti-Rabbit IgG (γ-chain specific)

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

mouse

共軛

biotin conjugate

抗體表格

purified immunoglobulin

抗體產品種類

secondary antibodies

無性繁殖

RG-96, monoclonal

形狀

buffered aqueous solution

物種活性

rabbit

應無反應活性

goat, feline, pig, guinea pig, rat, bovine, canine, human, horse, sheep, chicken

技術

direct ELISA: 1:150,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000
western blot (chemiluminescent): 1:200,000-1:400,000

同型

IgG1

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

IgG (immunoglobulin G) antibody is secreted by B cells and is found in blood and extracellular fluids and is enriched in mother′s milk. The variable region of IgG antibody is specific to antigens and is highly conserved.
The product recognizes an epitope located on the γ (heavy)-chain of rabbit IgG. In immunoblotting, the antibody recognizes both native and denatured forms of rabbit IgG. In ELISA, the antibody is specific for rabbit IgG, and shows no cross-reactivity with rabbit IgA and IgM or human IgG, IgA, and IgM. No cross reaction is observed with IgG from the following species: bovine, cat, chicken, dog, goat, guinea pig, horse, pig, rat, or sheep.

應用

Monoclonal Anti-Rabbit IgG (γ-chain specific)-Biotin is suitable for ELISA at a working dilution of 1:150,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. The antibody was used in β-catenin ELISA at a dilution of 1:2000.
Anti-Rabbit IgG (γ-chain specific)-Biotin antibody, Mouse monoclonal has been used in β-Catenin enzyme immunoassay. It has also been used in immunohistochemistry for examining histology of mammary glands.

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Rabbit IgG (γ-chain specific) antibody allows for the binding of various labels such as avidin or streptavidin. IgG antibody has its function similar to IgM antibody in complement system activation.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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