推荐产品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
WEN42, monoclonal
分子量
observed mol wt ~55 kDa
純化經由
using protein G
物種活性
rat
包裝
antibody small pack of 100
技術
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2a
表位序列
Extracellular domain
Protein ID登錄號
UniProt登錄號
儲存溫度
2-8°C
基因資訊
rat ... Clec4d(362432)
特異性
Clone WEN42 is a mouse monoclonal antibody that detects C-type lectin domain family 4 member D (CD368/CLEC4D). It targets an epitope within the extracellular domain.
免疫原
BWZ.36 cells expressing chimeric CD368/CLEC4D
應用
Quality Control Testing
Evaluated by Western Blotting with recombinant Rat CD368/CLEC4D protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant fragment of Rat CD368/CLEC4D expressed with Fc region of human IgG1.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD368/CLEC4D in Immunoprecipitation applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Immunohistochemistry Applications: A representative lot detected CD368/CLEC4D in Immunohistochemistry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406).
Western Blotting Analysis: A representative lot detected CD368/CLEC4D in Western Blotting applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Flow Cytometry Analysis: A representative lot detected CD368/CLEC4D in Flow Cytometry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant Rat CD368/CLEC4D protein.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant fragment of Rat CD368/CLEC4D expressed with Fc region of human IgG1.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD368/CLEC4D in Immunoprecipitation applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Immunohistochemistry Applications: A representative lot detected CD368/CLEC4D in Immunohistochemistry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406).
Western Blotting Analysis: A representative lot detected CD368/CLEC4D in Western Blotting applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Flow Cytometry Analysis: A representative lot detected CD368/CLEC4D in Flow Cytometry applications (Lobato-Pascual, A., et al. (2013). PLoS One. 8(2):e57406; Lobato-Pascual, A., et al. (2013). Eur J Immunol. 43(12):3167-74).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
C-type lectin domain family 4 member D (UniProt: Q69FH1; also known as C-type lectin superfamily member 8, CD368) is encoded by the Clec4d (also known as Clecsf8, Mcl) gene (Gene ID: 362432) in rat. CD368 is a membrane surface receptor that is expressed on blood monocytes and neutrophils, as well as on several tissue macrophage populations, including alveolar and peritoneal cavity macrophages. It is a single-pass type II protein that contains a cytoplasmic domain (aa 1-24), a transmembrane domain (aa 25-47), and an extracellular domain (aa 48-218). Its extracellular C-terminal region contains the C-type lectin-like domain (aa 89-206) that contains an evolutionarily conserved folded domain, and a carbohydrate recognition domain containing the Ca2+ binding sites. CD368 acts as a pattern recognition receptor (PRR) of the innate immune system that recognizes damage-associated molecular patterns (DAMPs) of pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. It can form disulfide-linked heterodimer with C-type lectin domain family 4 member E (CLEC4E) and with CLEC6A that forms a PPR against fungal infections. (Ref.: Lobato-Pascual, A., et al. (2013). PLoS ONE 8(2); e57406; Lobato-pascual, A., et al. (2013). Eur. J. Immunol. 43(12); 3167-3174).
外觀
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
重構
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommended storage: +2°C to +8°C .
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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