推荐产品
生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
F11, monoclonal
种属反应性
mouse, human
技术
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... SORT1(6272)
一般描述
Sortilin (UniProt Q99523; also known as 100 kDa NT receptor, Glycoprotein 95, Gp95, Neurotensin receptor 3, NT3, NTR3) is encoded by the SORT1 (also known as LDLCQ6, NT3) gene (Gene ID 6272) in human. Sortlin belongs to the family of sorting receptors that are characterized by the Vps10p domain, which creates a tunnel cavity with its ten-bladed β-propeller for the binding of soluble ligands. These family members also contain a short cytoplasmic tail that harbors recognition motifs for subcellular sorting adaptor proteins. Sortilin is reported to bind PCSK9 in the trans-Golgi network with high-affinity in HepG2 cells. Sort1 knockout mice exhibit low levels of PCSK9 in plasma and its subcellular localization in hepatocytes is also altered. Sortilin is also found to co-localize with amyloid precursor protein (APP) in neurites and it facilitates APP processing toward production of sAPPα, possibly by optimizing APP as substrate for α-secretase cleavage. Sortilin is initially produced with a signal (a.a. 1-33) and a propeptide (a.a. 34-77) sequence, the removal of which yields the mature receptor (a.a. 78-831) with a large luminal/extracellular (a.a. 78-555) portion, a transmembrane region(a.a. 756-778), and a short cytoplasmic tail (a.a.779-831). Like the other family members (MPRs and SorLA), sortilin resides both on the membrane of intracellular vesicles and on the plasma membrane. Altered sortilin expressions due to SORT1 genetic variations affect serum levels of low density lipoprotein cholesterol (LDL-C) and contribute to the LDL-C level quantitative trait locus 6 (LDLCQ6) and susceptibility to myocardial infarction.
免疫原
Epitope: Extracellular domain
Recombinant protein corresponding to the extracellular domain of human Sortilin.
应用
Anti-Sortilin Antibody, clone F11 is an antibody against Sortilin Antibody for use in Immunohistochemistry (Paraffin), Immunocytochemistry, Western Blotting and ELISA.
Immunocytochemistry Analysis: A representative lot detected cellular localization of endogenous sortilin in isolated hippocampal neurons from new born (P0) wild-type, but not Sort1−/− C57BL/6J mice (Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71).
Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318).
Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
Immunocytochemistry Analysis: Representative lots detected cellular localization of exogenously expressed sortilin in transfected HEK293 cells by fluorescent immunocytochemistry (Larsen, J.V., et al. (2014). Biochem J. 457(2):277-288; Gustafsen, C., et al. (2013). J Neurosci. 33(1):64-71; Larsen, J.V., et al. (2010). Mol. Cell. Biol. 30(17):4175-4187; Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
ELISA Analysis: A representative lot was employed as the detection antibody in sandwich ELISA. A positive correlation was found between PCSK9 and soluble sortilin in human serum samples from healthy individuals (Gustafsen, C., et al. (2014). Cell Metab. 19(2):310-318).
Western Blotting Analysis: A representative lot detected different distribution of Sortilin than SorLA among subcellular fractions obtained by gradient centrifugation (Nielsen, M.S., et al. (2007). Mol. Cell. Biol. 27(19):6842-6851).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Neuroscience
Developmental Neuroscience
质量
Evaluated by Immunohistochemistry in human cerebral cortex tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sortilin in human cerebral cortex tissue.
目标描述
~91 kDa calculated
外形
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
未找到合适的产品？
试试我们的产品选型工具.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Cyndia Charfi et al.
Frontiers in oncology, 11, 760787-760787 (2021-11-10)
Vasculogenic mimicry (VM) is defined as the formation of microvascular channels by genetically deregulated cancer cells and is often associated with high tumor grade and cancer therapy resistance. This microcirculation system, independent of endothelial cells, provides oxygen and nutrients to
Jean-Christophe Currie et al.
Cancers, 14(8) (2022-04-24)
Sortilin (SORT1) receptor-mediated endocytosis functions were exploited for this new approach for effective and safe treatments of gynecological cancers. Here, high expression of SORT1 was found in >75% of the clinically annotated ovarian and endometrial tumors analyzed by immunohistochemistry. Therefore
Peter Loof Møller et al.
Frontiers in cardiovascular medicine, 8, 652584-652584 (2021-05-04)
Genetic variants in the genomic region containing SORT1 (encoding the protein sortilin) are strongly associated with cholesterol levels and the risk of coronary artery disease (CAD). Circulating sortilin has therefore been proposed as a potential biomarker for cardiovascular disease. Multiple
Peder Madsen et al.
Scientific reports, 9(1), 611-611 (2019-01-27)
SorLA and Sortilin are multifunctional receptors involved in endocytosis and intracellular sorting of different and unrelated ligands. SorLA has recently attracted much attention as a novel strong risk gene for Alzheimer's disease, and much effort is currently being put into
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门