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生物源
rat
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
SUSI63B, monoclonal
分子量
calculated mol wt 144.44 kDa
observed mol wt ~145 kDa
純化經由
using protein G
物種活性
human, mouse
包裝
antibody small pack of 100 μL
技術
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG2aκ
表位序列
N-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
目標翻譯後修改
unmodified
基因資訊
mouse ... Pnpla3(116939)
一般說明
Cohesin subunit SA-1 (UniProt: Q9D3E6; also known as SCC3 homolog 1, Stromal antigen 1) is encoded by the Stag1 (also known as Sa1) gene (Gene ID: 20842) in murine species. Cohesin are known to mediate sister chromatid cohesion and 3D genome folding. They hold the sister chromatids together to ensure correct DNA repair by homologous recombination and proper chromosome segregation during cell division. They are essential for cell proliferation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped and at anaphase stage. The complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. This complex may also play a role in spindle pole assembly during mitosis. Two variant cohesin complexes containing Structural Maintenance of Chromosomes (SMC) 1, SMC3, RAD21 and either STAG/SA1 or STAG2/SA2 are present in all cell types. Before prophase, STAG1/SA1 appears to be scattered along chromosome arms. However, during prophase, most of cohesin complexes dissociate from chromatin. This dissociation is probably due to its phosphorylation by PLK1. At anaphase, the RAD21 subunit is cleaved that leads to the dissociation of the complex from chromosomes, allowing their separation. (Ref.: De Koninck, M., et al. (2020). Cell Rep.32(6); 108014; Kojic, A., et al. (2018). Nat. Struct. Mol. Biol. 25(6); 496-504).
特異性
Clone SUSI63B is a rat monoclonal antibody that detects STAG1/SA1. It targets an epitope within the N-terminal region.
免疫原
MBP-tagged recombinant fragment corresponding to the first 225 amino acids from N-terminal region of mouse Cohesin subunit SA-1 (STAG1).
應用
Quality Control Testing
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.
Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).
Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in MCF-7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.
Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).
Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
外觀
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
儲存和穩定性
Recommended storage: +2°C to +8°C.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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水污染物質分類（WGK）
WGK 1
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