MABE1057

Anti-ADA3 Antibody, clone 5C9/C8

clone 5C9/C8, from mouse

• 别名: Transcriptional adapter 3, ADA3 homolog, ADA3-like protein, Alteration/deficiency in activation 3, hADA3, STAF54, Transcriptional adapter 3-like
• UNSPSC代码: 12352203
• eCl@ss: 32160702

属性

• 生物来源: mouse
• 质量水平: 100
• 抗体形式: purified immunoglobulin
• 抗体产品类型: primary antibodies
• 克隆: 5C9/C8, monoclonal
• 种属反应性: human, mouse
• 技术: ChIP: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同位素/亚型: IgG1κ
• NCBI登记号: NP_006345
• UniProt登记号: O75528
• 运输: ambient
• 靶向翻译后修饰: unmodified
• 基因信息: human ... TADA3(10474)

说明

一般描述

Transcriptional adapter 3 (UniProt O75528; also known as ADA3 homolog, ADA3-like protein, Alteration/deficiency in activation 3, hADA3, STAF54, Transcriptional adapter 3-like) is encoded by the TADA3 (also known as ADA3, TADA3L) gene (Gene ID 10474) in human. Alteration/deficiency in activation 3 (ADA3) is a component of several transcriptional co-activator and histone acetyltransferase (HAT) complexes and plays a critical role in in cell cycle regulation. Ada3 deletion in mice is embryonic lethal, and Ada3-deficient mouse embryonic fibroblasts (MEFs) exhiit a severe proliferation defect, dramatic changes in global histone acetylation, mitotic defects, as well as a delay in G2/M-to-G1 and G1-to-S transition. ADA3 also plays a role in genomic stability by controlling DNA repair checkpoints. ChIP-seq analsis reveals that ADA3 is significantly associated with human centromere regions across most chromosomes. In addition, ADA3 is found associated with CENP-B throughout all phases of the cell cycle, and CENP-B centromere binding decreased upon ADA3 knockdown. Wild-type human ADA3, but not CENP-B-binding deficient ADA3 mutant, prevented cell proliferation defects in MEFs following endogenous mouse ADA3 knockown. ADA3 overexpression and mislocalization correlates with poor prognosis in breast cancer patients.

特异性

Clone 5C9/C8 specifically detected Cre recombinase expression-induced ADA3 downregulation in Ada3FL/FL MEFs. Clone 5C9/C8 immunostained the nuclei of untransfected, but not ADA3 shRNA-transfected, 76N-TERT human mammary epithelial cells (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310; Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).

免疫原

Full-length recombinant human ADA3.

应用

Anti-ADA3, clone 5C9/C8, Cat. No. MABE1057, is a highly specific mouse monoclonal antibody that targets TADA3 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected nuclear ADA3 immunoreactivity in formalin-fixed, paraffin-embedded human breast carcinoma tissue section (Courtesy of Vimla Band, Ph.D., University of Nebraska USA).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected ADA3 occupancy at the X chromosome centromere HOR region, the kinetochore assembly site also occupied by CENP-A and CENP-B, using TERT-immortalized human mammary epithelial cell 76N-TERT chromatin preparation. shRNA-mediated ADA3 knockdown led to a reduction in CENP-B, but not CENP-A, recruitment to the HOR region (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310).

Immunocytochemistry Analysis: A representative lot detected ADA3 nuclear immunoreactivity in untransfected, but not ADA3 shRNA-transfected, TERT-immortalized human mammary epithelial 76N-TERT cells by fluorescent immunocytochemistry staining of 4% formaldehyde-fixed cells. Dual fluorescent staining revealed ADA3 and CENP-B nuclear colocalization (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310).

Western Blotting Analysis: Representative lots detected both the endogenous mouse ADA3 (mADA3) and the exogenously expressed human ADA3 (hADA3) in Ada3FL/FL MEFs virally infected to express FLAG-tagged full-length or a.a. 111-432 hADA3 constructs. Cre recombinase expression downregulated only mADA3, but not hADA3 (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310; Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).

Western Blotting Analysis: A representative lot detected ADA3 expression levels among a panel of mouse tissues (Mohibi, S., et al. (2012). J. Biol. Chem. 287(35):29442-29456).

Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ADA3 in 10 µg of MCF-7 cell lysate.

目标描述

~55 kDa observed. 48.90 kDa (human and mouse isoform 1) calculated. The larger-than-calculated band size is consistent with that reported in the literature (Mohibi, S., et al. (2015). J. Biol. Chem. 290(47):28299-28310). Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.

Format: Purified

Purified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable