MAB1997-AF647

Anti-Integrin β1 Antibody, clone MB1.2 Antibody, Alexa Fluor^™ 647 Conjugate

clone MB1.2, from rat, ALEXA FLUOR^™ 647

• 别名: Integrin beta-1, CD29, Fibronectin receptor subunit beta, GpIIa, VLA-1/2/3/4/5/6 subunit beta
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

属性

• 生物源: rat
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 647
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: MB1.2, monoclonal
• 物種活性: mouse
• 技術: flow cytometry: suitable; immunocytochemistry: suitable
• 同型: IgG2aκ
• NCBI登錄號: NP_034708
• UniProt登錄號: P09055
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Itgb1(16412)

说明

一般說明

Integrin beta-1 (UniProt P09055; also known as CD29, Fibronectin receptor subunit beta, GpIIa, VLA-1/2/3/4/5/6 subunit beta) is encoded by the Itgb1 (also known as Fnrb) gene (Gene ID 16412) in murine species. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

特異性

The specificity of MB1.2 was determined by its binding to antigen, and the ability of antiserum against the carboxyl terminal of VLA-beta 1 subunit to deplete antigens for MB1.2 in sequential immunoprecipitation experiments. MB1.2 had no effect on cell adhesion to matrix proteins (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).

免疫原

Mouse Integrin beta-1 (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).

應用

Anti-Integrin 1, clone MB1.2, Alexa Fluor^™ 647 Conjugate, Cat. No. MAB1997-AF647, is a highly specific rat monoclonal antibody that targets Integrin 1 and has been tested in Flow Cytometry and Immunocytochemistry.

Flow Cytometry Analysis: 0.2 µL from a representative lot detected Integrin 1 on the surface of one million NIH/3T3 cells.

The unconjugated antibody (Cat. No. MAB1997) and FITC conjugate (Cat. No. FCMAB269F) are also available for Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting applications.

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Immunocytochemistry in NIH/3T3 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Integrin-beta1 in NIH/3T3 cells.

標靶描述

~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).

外觀

Protein G purified.

Purified rat IgG2a Alexa Fluor^™ 647 conjugate in buffer containing PBS, 1.5% BSA with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable