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Merck

935409

Sigma-Aldrich

Sulfo-NHS-LC-LC-Biotin

≥95%

别名:

3-Pyrrolidinesulfonic acid, 1-[[6-[[6-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]amino]-1-oxohexyl]amino]-1-oxohexyl]oxy]-2,5-dioxo-, monosodium salt, [3aS-(3aα,4β,6aα)]-[partial]-, EZ-Link Sulfo-NHS-LC-LC-Biotin, Hexanoic acid, 6-[[6-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]-1-oxohexyl]amino]-, 2,5-dioxo-3-sulfo-1-pyrrolidinyl ester, sodium salt (1:1), Sulfo-NHS-XX-Biotin

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About This Item

经验公式(希尔记法):
C26H40N5O10S2·Na
分子量:
669.74
MDL號碼:
分類程式碼代碼:
12352100
NACRES:
NA.21

品質等級

化驗

≥95%

形狀

solid

儲存溫度

−20°C

SMILES 字串

[Na].O=C1NC2CSC(CCCCC(=O)NCCCCCC(=O)NCCCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)O)C2N1

InChI

InChI=1S/C26H41N5O10S2.Na/c32-20(27-14-8-2-4-12-23(35)41-31-22(34)15-19(25(31)36)43(38,39)40)10-3-1-7-13-28-21(33)11-6-5-9-18-24-17(16-42-18)29-26(37)30-24;/h17-19,24H,1-16H2,(H,27,32)(H,28,33)(H2,29,30,37)(H,38,39,40);/t17-,18-,19?,24-;/m0./s1

InChI 密鑰

RWNPTJCDHAXLGI-BJPAGVOZSA-N

應用

Sulfo-NHS-LC-LC-biotin finds extensive applications in scientific research for the purpose of protein labeling and detection. Its utilization in protein purification allows for the isolation of biotinylated proteins from intricate mixtures. Moreover, it plays a vital role in protein-protein interaction studies, enabling investigations into the binding affinity and specificity of proteins. Additionally, it serves as a valuable tool in protein quantification, facilitating the measurement of specific protein levels within a given sample.

特點和優勢

Sulfo-NHS-LC-LC-biotin is a frequently employed chemical compound in scientific investigations aimed at examining and characterizing proteins. This compound serves as a biotinylation agent, allowing for the labeling of proteins in diverse applications, including protein purification, studies on protein-protein interactions, and protein quantification. Sulfo-NHS-LC-LC-biotin works by reacting with the primary amines of proteins to form stable amide bonds. This results in the biotinylation of the protein, which allows for the detection and isolation of the protein using streptavidin or avidin conjugated to a detection system, such as a fluorescent or radioactive label.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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D Castelletti et al.
Clinical and experimental immunology, 136(2), 365-372 (2004-04-17)
Hodgkin's lymphoma patients treated with an anti-CD25 Ricin toxin A-chain (RTA)-based Immunotoxin (RFT5.dgA) develop an immune response against the toxic moiety of the immunoconjugate. The anti-RTA antibody response of 15 patients showing different clinical features and receiving different total amounts
M Ichikawa et al.
Bioorganic & medicinal chemistry letters, 11(13), 1769-1773 (2001-06-27)
We have prepared several mechanism-based affinity-labeling agents for possible use in isolating N-acetylglucosaminidase, in which an N-acetylglucosamine is linked to an o-monofluoro- or difluoro-methyl phenoxy glycoside with or without a cleavable disulfide group in the tether to biotin.
A reagent for covalently attaching biotin to proteins via a cleavable connector arm.
C A Mouton et al.
Archives of biochemistry and biophysics, 218(1), 101-108 (1982-10-01)
Chemical probes of extended biological structures: synthesis and properties of the cleavable protein cross-linking reagent [35S]dithiobis(succinimidyl propionate).
A J Lomant et al.
Journal of molecular biology, 104(1), 243-261 (1976-06-14)

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