HPLC Analysis of Glutamic Acid Enantiomers on Astec^® CHIROBIOTIC^® T (23 °C)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (20:80:0.02, A:B:C)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 205 nm

injection

5 μL

sample

1 mg/mL in methanol