HPLC Analysis of Tricyclic Antidepressants on Ascentis® ES Cyano
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Amitriptyline hydrochloride solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®N-Desmethyldoxepin-D3 hydrochloride solution
cis/trans, 100 μg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Desipramine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Doxepin hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Imipramine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Nortriptyline hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Protriptyline hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis ES Cyano, 15 cm x 4.6 mm I.D., 5 μm particles (577306-U)
mobile phase
[A] 10 mM potassium phosphate dibasic, pH 7 with phosphoric acid; [B] acetonitrile; [C] methanol; (25:60:15, A:B:C)
flow rate
2 mL/min
column temp.
25 °C
detector
UV, 215 nm
injection
5 μL
sample
100 μg/mL in water: methanol (70:30)
Description
Analysis Note
This application demonstrates the suitability of Ascentis ES Cyano for the efficient separation of tricyclic antidepressants.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany