HPLC Analysis of Peptides on Proteomix® SCX-NP3, Volatile Buffer, ELSD and UV Detection
Materials
analytical column
Product No.
Description
Pricing
Proteomix® SCX-NP3 Strong Cation Exchange Column
L × I.D. 5 cm × 4.6 mm, 3 μm particle size, stainless steelCONDITIONS
column
Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)
mobile phase
(A) 5 mM ammonium acetate; (B) 50 mM ammonium acetate:acetonitrile (4:1, v:v)
gradient
0 to 50% B in 20 min
flow rate
0.6 mL/min
detector
ELSD (SofTA) and UV, 280 nm
injection
5 μL
sample
0.1 mg/mL
Description
Legal Information
Proteomix is a registered trademark of Sepax Technologies