HPLC Analysis of Peptide Antibiotics on Discovery^® C8

column

Discovery C8, 15 cm × 4.6 mm I.D., 5 μm particles (59353)

mobile phase

[A] 25mM potassium phosphate, pH 3; [B] acetonitrile, (60:40, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL 10 μL