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Synthesis of YF3: Yb, Er upconverting nanofluorophores using chitosan and their cytotoxicity in MCF-7 cells.

Synthesis of YF3: Yb, Er upconverting nanofluorophores using chitosan and their cytotoxicity in MCF-7 cells.

International journal of biological macromolecules (2014-12-03)

Sethuraman Gayathri, Oriparambil Sivaraman Nirmal Ghosh, Annamraju Kasi Viswanath, P Sudhakara, M Jeevan Kumar Reddy, A M Shanmugharaj

ABSTRACT

In this work, we described one pot hydrothermal synthesis of surface modified water soluble YF3: Yb, Er upconverting nanofluorophores (UCNFs) using natural biopolymer chitosan. The obtained nanocrystals have undergone X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), high resolution transmission electron microscopy (HRTEM) and photoluminescence (PL) studies. The nanoparticles possess uniform particle size distribution with average size about 27 nm. The cytotoxicity results revealed that the chitosan capped nanoparticles exhibit excellent biocompatibility in human breast cancer cells. In conclusion, the water soluble chitosan capped YF3: Yb, Er nanoparticles could be used as a potential candidate in bio-imaging and therapeutic applications.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ytterbium, powder, ≥99.9% trace rare earth metals basis

Sigma-Aldrich

Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)

Sigma-Aldrich

Ethanol, JIS special grade, 94.8-95.8%

Sigma-Aldrich

Ammonium fluoride, ≥99.99% trace metals basis

Sigma-Aldrich

Ethanol, JIS first grade, 94.8-95.8%

Ytterbium, foil, not light tested, 50x50mm, thickness 0.025mm, as rolled, 99%

Ytterbium, lump, 25g, max. size 25mm, 99.9%

Ytterbium, foil, not light tested, 25x25mm, thickness 0.005mm, as rolled, 99%

Ytterbium, rod, 50mm, diameter 6.35mm, cast, 99%

Ytterbium, lump, 100g, max. size 25mm, 99.9%

Ytterbium, foil, not light tested, 25x25mm, thickness 0.025mm, as rolled, 99%

Ytterbium, rod, 100mm, diameter 6.35mm, cast, 99%

Ytterbium, lump, 50g, max. size 25mm, 99.9%

Ytterbium, rod, 50mm, diameter 10.0mm, cast, 99%

Ytterbium, rod, 50mm, diameter 2.0mm, cast, 99%

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for HPLC

Sigma-Aldrich

Ethanol, ≥99.5%, SAJ super special grade

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Ethanol, ≥99.5%, suitable for absorption spectrum analysis

Sigma-Aldrich

Ethanol, JIS 300, ≥99.5%, for residue analysis

Sigma-Aldrich

Ethanol, JIS 1000, ≥99.5%, for residue analysis

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Yttrium(III) chloride, anhydrous, powder, 99.99% trace metals basis

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

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Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, meets USP testing specifications

Sigma-Aldrich

Yttrium(III) chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

Supelco

Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

Sigma-Aldrich

Ethanol, ≥99.5%

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for fluorescence

Erbium, rod, 100mm, diameter 6.35mm, cast, 99%

Erbium, foil, 25x25mm, thickness 0.125mm, as rolled, 99%