Skip to Content
Merck
  • Validated UPLC-MS/MS method for simultaneous determination of simvastatin, simvastatin hydroxy acid and berberine in rat plasma: Application to the drug-drug pharmacokinetic interaction study of simvastatin combined with berberine after oral administration in rats.

Validated UPLC-MS/MS method for simultaneous determination of simvastatin, simvastatin hydroxy acid and berberine in rat plasma: Application to the drug-drug pharmacokinetic interaction study of simvastatin combined with berberine after oral administration in rats.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2015-11-01)
Mei Liu, Xianying Su, Guofei Li, Guilian Zhao, Limei Zhao
ABSTRACT

A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) assay method was developed and validated for simultaneous quantification of simvastatin (SV), its metabolite simvastatin hydroxy acid (SVA) and berberine (BBR) in rat plasma. Separation was performed on Poroshell 120 EC-C18 column (4.6×50mm, 2.7μm) using gradient elution by mobile phase containing acetonitrile and 10mM ammonium acetate (pH 4.5). Polarity switch (positive-negative-positive ionization mode) was performed in a total run time of 4.0min. The lower limits of quantification (LLOQ) for SV, SVA and BBR were 0.10, 0.20 and 0.10ng/mL, respectively. The response function was established for concentration range of 0.10-100ng/mL for SV and BBR and 0.20-3000ng/mL for SVA, with a coefficient of correlation of >0.99 for all the compounds. The proposed method was applied to the drug-drug pharmacokinetic interaction study of SV combined with BBR after oral administration in rats.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, 99.999% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate solution, 50 % (w/v), for copper determination
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, BioXtra, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate solution, for molecular biology, 7.5 M
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, reagent grade, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, ACS reagent, ≥97%
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, ≥99.99% trace metals basis
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, Laboratory Reagent, ≥99.6%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, Absolute - Acetone free
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, NMR reference standard
Pricing and availability is not currently available.
Sigma-Aldrich
Ammonium acetate, for molecular biology, ≥98%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
Pricing and availability is not currently available.
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, SAJ special grade
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, 99.93%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
Sigma-Aldrich
Methanol, ACS spectrophotometric grade, ≥99.9%
Pricing and availability is not currently available.