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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ABSTRACT

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Acetone, ≥99.5%, for residue analysis

Sigma-Aldrich

Acetone, JIS special grade, ≥99.5%

Sigma-Aldrich

Acetone, SAJ first grade, ≥99.0%

Sigma-Aldrich

Acetone, for residue analysis, JIS 5000

Sigma-Aldrich

Acetone, suitable for HPLC

Sigma-Aldrich

Acetone, for chromatography, ≥99.8%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 53.5-56.5% in H₂O

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

2-Bromo-2-methylpropionic acid, 98%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 1.0 M in methanol

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Copper(II) bromide, 99.999% trace metals basis

Sigma-Aldrich

Copper(II) bromide, 99%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 40 wt. % in H₂O

Sigma-Aldrich

Acetone, for residue analysis, ≥99.5%

Sigma-Aldrich

Acetone, histological grade, ≥99.5%

Sigma-Aldrich

Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

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Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

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Acetone, ACS reagent, ≥99.5%

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Acetone, Laboratory Reagent, ≥99.5%

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L-Glutamine

Sigma-Aldrich

Dimethyl sulfoxide solution, 50 wt. % in H₂O

Sigma-Aldrich

Dimethyl sulfoxide, suitable for HPLC

Sigma-Aldrich

1,2-Dichloroethane, JIS special grade, ≥99.5%

Sigma-Aldrich

Diethylamine, SAJ special grade, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, SAJ first grade, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5%

Sigma-Aldrich

Ethylenediamine, SAJ special grade, ≥99.0%