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Merck

Novel compound for identifying Escherichia coli.

Applied and environmental microbiology (1988-07-01)
W D Watkins, S R Rippey, C R Clavet, D J Kelley-Reitz, W Burkhardt
ABSTRACT

A new chromogenic compound, 5-bromo-4-chloro-3-indoxyl-beta-D-glucuronide, was found to be useful for the rapid, specific, differential identification of Escherichia coli in the sanitary analysis of shellfish and wastewater. Of 1,025 presumptively positive colonies (blue) and 583 presumptively negative colonies (nonblue), only 1% false-negative and 5% false-positive results were found.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
5-Bromo-4-chloro-3-indolyl β-D-glucopyranoside, ≥97%