- Temperature-controlled assembly of high ordered/disordered dodecylamine layers on HOPG: consequences for DNA patterning.
Temperature-controlled assembly of high ordered/disordered dodecylamine layers on HOPG: consequences for DNA patterning.
Langmuir : the ACS journal of surfaces and colloids (2009-05-14)
Jozef Adamcik, Susana Tobenas, Giovanni Di Santo, Dmitry Klinov, Giovanni Dietler
PMID19437780
ABSTRACT
It is shown that temperature-controlled ordered layers of dodecylamine, self-assembled on highly oriented pyrolytic graphite (HOPG), are an appropriate substrate for aligning individual DNA molecules. High resolution atomic force microscopy (AFM) permits visualization of the lamellar structure of dodecylamine on HOPG. The DNA adsorbed on ordered dodecylamine layers is stretched and oriented along the lamellae, while DNA on disordered dodecylamine layers is found to be in a random conformation. Small DNA bubbles appear in the case of partially denaturated linear double stranded DNA (dsDNA) adsorbed on ordered layers of dodecylamine.