Detergent-based Western Blot Stripping Buffer
Stripping and re-probing a Western blot enables sample conservation and fast correction or adjustments of antibody concentration and incubation time. This recipe is for a Western blot stripping solution that uses the surfactant sodium dodecyl sulfate (SDS) and the reducing agent β-mercaptoethanol (BME) to remove antibodies and chemiluminescent or fluorescent detection reagents.
This calculator enables the accurate preparation of this detergent-based Western blot stripping solution for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.
Detergent-based Western Blot Stripping Solution Recipe Calculator
| 100 mL Recipe | Desired Volume: mL |
| 20 mL of 10% SDS (71736) | mL of 10% SDS |
| 12.5 mL of 0.5 M Tris-HCl pH 6.8 (FW 157.60) | mL of 0.5 M Tris HCl pH 6.8 |
| 0.8 mL of β-mercaptoethanol (63689) | mL of β-mercaptoethanol |
| Adjust final volume to 100 mL with Milli-Q® Water | Adjust final volume to mL with Milli-Q® Water |
| Procedure | |
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OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS
Stock Solutions
- 4X SDS Sample Loading Buffer for Western Blotting
- 10X Phosphate-Buffered Saline (PBS)
- 10X Tris-Buffered Saline (TBS) Stock Solution
- Stock Components for Western Blot Stripping Solutions
Wash & Blocking Buffers
- 1X Tris-Buffered Saline (TBS) Working Solution
- 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
- 1X PBS (Phosphate-Buffered Saline)
- 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
- Non-Fat Dry Milk (NFDM) Blocking Buffer
- Bovine Serum Albumin (BSA) Blocking Buffer
Stripping Solutions
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