ZRB1910
Anti-ApoE Antibody, clone 3N14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Apo-E
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3N14, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 36 kDa
purified by
using Protein A
species reactivity
human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... APOE(348)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 3N14 is a ZooMAb® rabbit recombinant monoclonal antibody that detects ApoE. It targets an epitope within 11 amino acids from the N-terminal half of the protein.
Immunogen
KLH-conjugated linear peptide corresponding to 11 amino acids from the N-terminal half of Human ApoE.
Application
Quality Control Testing
Evaluated by Immunocytochemistry in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected ApoE in HepG2 cells.
Tested Applications
Affinity Binding Assay: The binding affinity of this antibody to ApoE protein was at least one thousand-fold (1,000X) higher than that obtained with the non-specific GST control protein.
Flow Cytometry Analysis: 1 µg from a representative lot detected ApoE in one million HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Immunocytochemistry in HepG2 cell lysate.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected ApoE in HepG2 cells.
Tested Applications
Affinity Binding Assay: The binding affinity of this antibody to ApoE protein was at least one thousand-fold (1,000X) higher than that obtained with the non-specific GST control protein.
Flow Cytometry Analysis: 1 µg from a representative lot detected ApoE in one million HepG2 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Apolipoprotein E (ApoE) (UniProt: P02649; also known as Apo-E) is encoded by the APOE gene (Gene ID: 348) in human. ApoE plays a crucial role in lipid metabolism and transport. It binds to lipoprotein receptors and facilitates the uptake and clearance of lipoproteins, such as low-density lipoprotein (LDL), from the bloodstream. ApoE is involved in the redistribution of cholesterol and other lipids among various tissues and organs. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. ApoE has been associated with various diseases. The APOE4 isoform, in particular, has been linked to an increased risk of developing earlier onset Alzheimer′s disease. APOE has also been implicated in cardiovascular diseases, such as atherosclerosis and coronary heart disease. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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