MABS1897

Anti-PQBP1 Antibody, clone 7A1.1

clone 7A1.1, from mouse

• Synonym(s): Polyglutamine-binding protein 1, PQBP-1, 38 kDa nuclear protein containing a WW domain, Npw38, Polyglutamine tract-binding protein 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 7A1.1, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001027553
• UniProt accession no.: O60828

Description

General description

Polyglutamine-binding protein 1 (UniProt: O60828; also known as PQBP-1, 38 kDa nuclear protein containing a WW domain, Npw38, Polyglutamine tract-binding protein 1) is encoded by the PQBP1 (also known as NPW38) gene (Gene ID: 10084) in human. PQBP-1 is an intrinsically disordered protein that acts as a scaffold and is involved in different processes, such as pre-mRNA splicing, transcription regulation, innate immunity and neuron development. It is widely expressed with high levels in heart, skeletal muscle, pancreas, spleen, thymus, prostate, ovary, and small intestine. It is also reported to be expressed in leukocytes. PQBP-1 interacts with splicing-related factors via the intrinsically disordered region and regulates alternative splicing of target pre-mRNA species. It is also reported to suppress the ability of POU3F2 to transactivate the DRD1 gene in a POU3F2 dependent manner. It is shown to interact with ATXN1 and this correlates positively with the length of the polyglutamine tract. It also interacts with RNA polymerase II large subunit in a phosphorylation-dependent manner and forms a ternary complex with ATXN1 mutant and phosphorylated RNA polymerase II. It may also be involved in ATXN1 mutant-induced cell death and the interaction with ATXN1 mutant reduces levels of phosphorylated RNA polymerase II large subunit. PQBP-1 contains a WW domain (aa 46-80), which plays a role as a transcriptional activator directly or via association with the transcription machinery. The WW domain also mediates interaction with WBP11, ATN1, SF3B1 and the C-terminal domain of the RNA polymerase II large subunit. Mutations in PQBP1 gene have been linked to Renpenning syndrome 1, which is has been characterized by mental retardation, microcephaly, short stature, and small testes.

Specificity

Clone 7A1.1 specifically detects human Polyglutamine-binding protein 1. It targets an epitope with in 15 amino acids from the internal regions.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Polyglutamine-binding protein 1.

Application

Anti-PQBP1, clone 7A1.1, Cat. No. MABS1897, is a mouse monoclonal antibody that detects Polyglutamine-binding protein 1 and has been tested for use in and Immunohistochemistry (Paraffin) and Western Blotting..

Immunohistochemistry (Paraffin) Analysis: A 1:250 and 1:50 dilution from a representative lot detected PQBP1 in human cerebral cortex and human pancreas tissue sections, respectively.

Quality

Evaluated by Western Blotting in HEPG2 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected PQBP1 in HEPG2 cell lysate.

Target description

~38 kDa observed; 30.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.