MABF2658

Anti-TIGIT Antibody, clone 4D4

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: hamster
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4D4, monoclonal
• mol wt: calculated mol wt 26.96 kDa; observed mol wt ~50 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100 μL
• technique(s): ELISA: suitable; flow cytometry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Unknown
• Protein ID accession no.: Unknown
• UniProt accession no.: P86176
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Tigit(100043314)

Description

General description

T-cell immunoreceptor with Ig and ITIM domains (UniProt: P86176; also known as V-set and immunoglobulin domain-containing protein 9, TIGIT) is encoded by the Tigit (also known as Vstm3) gene (Gene ID: 100043314) in murine species. TIGIT is a single-pass type I, homodimeric, membrane protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 29-148), a transmembrane domain (aa 149-169), and a cytoplasmic domain (aa 170-249). The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 234-239), which is involved in the modulation of its cellular responses. It serves as a coinhibitory receptors with CD226 and is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells and blocks immune responses by affecting T cells and APCs. It is shown to bind to poliovirus receptor (PVR) with high affinity to form a heterotetrameric assembly of two TIGIT and two PVR molecules. This causes increased secretion of IL-10 and decreased secretion of IL-12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT also serves as a functional ligand and induces dendritic cells to acquire a tolerogenic phenotype via ligation of PVR. TIGIT -/- mice, when challenged, develop severe experimental autoimmune encephalomyelitis compared to wild-type mice. Loss of TIGIT also in mice also results in hyperproliferative T cell responses and increased susceptibility to autoimmunity. (Ref.: Dixon, KO., et al. (2018). J. Immunol. 200(8); 3000-3007; Joller, N., et al. (2011). J. Immunol. 186(3); 1338-1342).

Specificity

Clone 4D4 is an Armenian Hamster monoclonal antibody that detects murine T-cell immunoreceptor with Ig and ITIM domains (TIGIT).

Immunogen

Recombinant mouse TIGIT tetramers.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant mouse TIGIT-Fc chimera protein.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant mouse Fc-His-tagged TIGIT chimera protein.

Tested Applications

ELISA Analysis: A representative lot detected TIGIT in ELISA applications (Joller, N., et al. (2011). J Immunol. 186(3):1338-42).

ELISA Analysis: Various dilutions from a representative lot detected Fc-His-tagged TIGIT chimera protein .

Flow Cytometry Analysis: A representative lot detected TIGIT in Flow Cytometry applications (Joller, N., et al. (2011). J Immunol. 186(3):1338-42).

Agonist Action: A representative lot of this antibody reduced proliferation of T cells stimulated with Anti-CD3/Anti-CD28 beads in vitro. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).

Inhibition Assay: A representative lot of this antibody fully blocked CD155 binding to TIGIT. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TIGIT, clone 4D4, Cat. No. MABF2658, is an Armenian hamster monoclonal antibody that detects TIGIT and is tested for use in ELISA, Flow Cytometry, Agonist Action, Inhibition Assay, and Western Blotting.

Physical form

Purified Armenian hamster monoclonal antibody IgG in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable