HPLC Analysis of Trypsin on TSKgel^® Butyl-NPR

column

TSKgel Butyl-NPR, 3.5 cm × 4.6 mm I.D., 2.5 μm particles (814947)

mobile phase

2 M to 0 M ammonium sulfate in 20 mM Tris-HCI buffer, pH 7.5 in 10 min

flow rate

1 mL/min

detector

UV, 280 nm

injection

commercial trypsin sample