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HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode)

HPLC Analysis of Polar Compounds on Ascentis® Express OH5 (HILIC Mode) application for HPLC

Materiales

Estándar

Referencia del producto
Descripción
Precios

C1778

Caffeine

Sigma Reference Standard, vial of 250 mg

C5901

Codeine monohydrate

analytical standard

columna analítica

Referencia del producto
Descripción
Precios

53769-U

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 3 mm

CONDITIONS

column

Ascentis Express OH5, 10 cm x 3 mm I.D., 2.7 μm particles (53769-U)

mobile phase

5 mM ammonium formate (95:5 acetonitrile:water) pH 6.8

flow rate

0.6 mL/min

pressure

965 psi (66.5 bar)

column temp.

30 °C

detector

UV, 254 nm

injection

1 μL

sample

100 μg/mL in 25:75, water:methanol

Descripción

Nota de análisis

The impact of partitioning and ion exchange in HILIC separations is demonstrated using a mixture of acidic, neutral, and basic polar compounds in this Figure. All three analytes have similar log D values, both acid and base are ionized at mobile phase pH.

Información legal

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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