HPLC Analysis of Miconazole Enantiomers on Astec^® CYCLOBOND I 2000 RSP

column

CYCLOBOND I 2000 RSP, 25 cm x 4.6 mm I.D., 5 μm particles (20324AST)

mobile phase

[A] 1% triethylamine acetate, pH 4.1; [B] acetonitrile; (80:20, A:B)

flow rate

2 mL/min

column temp.

ambient

detector

UV, 230 nm

injection

5 μL

sample

5 mg/mL in methanol