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HPLC Analysis of Insulin (Species Variants) on BIOshell A160 Peptide C18

HPLC Analysis of Insulin (Species Variants) on BIOshell A160 Peptide C18 application for HPLC

Materiales

Analytical column

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Descripción
Precios

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Standard

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Descripción
Precios

Insulin from porcine pancreas

powder, ≥27 USP units/mg

Insulina from bovine pancreas

≥25 USP units/mg (HPLC), powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 70:30, water with 0.1% TFA:acetonitrile with 0.09% TFA; [B] 55:45, water with 0.1% TFA:acetonitrile with 0.09% TFA

gradient

0 to 100% B in 7.5 min

flow rate

0.3 mL/min

pressure

4040 psi (278 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

0.5 μL

sample

200 μg/mL insulin (bovine, human, porcine) in 0.1% formic acid

Descripción

Analysis Note

These three insulins have more than 76% identical positions in a primary structure alignment. The human and porcine proteins are most similar with 85% identity. Baseline resolution of these insulins in achieved on BIOshell A160 Peptide C18, with relative band spacing of these three peaks approximating their relative sequence similarities. BIOshell A160 Peptide C18 is ideally suited for RP chromatography of small proteins or large peptides.

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