HPLC Analysis of Folinic Acid Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] methanol; [B] acetic acid; [C] triethylamine; (100:1:0.5, A:B:C)

flow rate

2 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

2 μL

sample

5 mg/mL in methanol