HPLC Analysis of Aflatoxins on Ascentis^® Express C18

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 5 mM ammonium formate, pH 2.5 (titrated with formic acid); [B] methanol

gradient

5 to 100% B in 10 min

flow rate

0.4 mL/min

pressure

4714 psi (325 bar)

column temp.

35 °C

detector

APCI (+)

injection

5 μL

sample

0.15 - 30 mg/L in 90:10, water:acetonitrile