GC Analysis of Organophosphorus Pesticides (OP-Pest) on SLB^®-5ms after SPME using 60 μm Carbowax^® Fiber

sample/matrix

Organophosphate pesticides at 10ppb in 0.2M phasphate buffer, pH 7, with 25% NaCl, 9mL in 10 mL vial

SPME fiber

60 μm Carbowax(PEG) (57354-U)

extraction

30 min. direct immersion with agitation using CombiPal autosampler

desorption process

2 min at 250°C, splitless for 0.75 min then opened

column

SLB-5ms 30 m x 0.25mm I.D., 0.5 μm film

oven

60°C(1 min) to 215°C @ 20°C/min to 290°C @ 6°C/min (1 min)

inj. temp.

Splitless/split with 0.75mm ID liner and Merlin Microseal

detector

ITMS m/z=50-326 @ 0.6 μsec per scan, transfer line 310°C

carrier gas

Helium, 1mL/min constant flow